Composite

Part:BBa_K1736200:Design

Designed by: Nick Coleman   Group: iGEM15_Sydney_Australia   (2015-09-17)

Harmonised Ethene Monooxygenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 554
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 119
    Illegal BglII site found at 3698
    Illegal BamHI site found at 254
    Illegal BamHI site found at 969
    Illegal BamHI site found at 1887
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 146
    Illegal NgoMIV site found at 1717
    Illegal NgoMIV site found at 2286
    Illegal NgoMIV site found at 2825
    Illegal NgoMIV site found at 3246
    Illegal AgeI site found at 3949
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Four ORFs: etnA, etnB, etnC, etn D, codon-optimised for expression in Pseudomonas putida, each ORF has native RBS upstream

Source

Harmonised sequence (via codon harmonisation algorithm [http://2015.igem.org/Team:Sydney_Australia/TransOpt TransOpt]) of the Mycobacterium smegmatis ethene monooxygenase enzyme for expression in Pseudomonas.

References